Chang, Emmanuel

Professor and Chair

Chemistry

Phone: 718-262-3778
Office Location: AC-3F01G
Email: echang@york.cuny.edu

Research in the lab of Emmanuel Chang centers around biological applications of mass spectrometry. Our major interest is in analysis and methods development for protein phosphorylation and other post-translational modifications. Applications include phosphorylation in cell cycle; ADP-ribosyltaion; mass spectrometry-based biosensors, and kinase inhibitors and enzyme kinetics. We also collaborate with other CUNY and external scientists who would like to harness the power of mass spectrometry to study there own interesting biological systems.

We supplement our experimental work with computational methods including bioinformatic protein sequence analysis and molecular modeling.

Dr. Chang's teaching interests include analytical chemistry, biochemistry, writing and data presentation, literature analysis, and innovations in introductory chemistry.

Curriculum Vitae

Office Hours

Day	Time
Monday	2:00 PM - 2:50 PM
Wednesday	11:00 AM - 11:50 AM

Education

Degree	Institution	Field	Dates
PhD	The Rockefeller University	Biochemistry	2006
BA	Princeton University	Chemistry	1999

Areas of Expertise

Biochemistry
Analytical Chemistry
Mass Spectrometry
Proteomics
Bioinformatics
Phosphorylation
Cell Cycle
Chemical and Biochemical
Chemical Education
Introductory Chemistry
Instrumental Analysis

Publications in Field Of Expertise

Articles

Dhital B, Durlik P, Rathod P, Gul-E-Noor F, Wang Z, Sun C, Chang EJ, Itin B, Boutis GS. "Ultraviolet Radiation Reduces Desmosine Cross-links in Elastin." Biochemical and Biophysical Reports. 10 2017: 172.

Abdullah R, Patil KS, Pal R, Prabhudesai S, Lee S, Yang P, Panik K, Hoedt E, Basak I, Ho H-P, Chang EJ, Tzoulis C, Alves G, Neubert TA, Larsen JP, and Moller SG. "Subcellular alpha-synuclein Species Show Altered Behavior in Neurodegeneration." Molecular Neurobiology. 54 2017: 7639.

Vedad J, Domarodzki M, Mojica E-RE, Chang EJ, Profit AA, Desamero RZB. "Conformational Differentiation of alpha-cyanohydroxycinnamic acid isomers: a Raman spectroscopic study." Journal of Raman Spectroscopy. 48 2017: 1282.

Patil KS, Basak I, Pal R, Alves G, Ho H-P, Chang EJ, Larsen JP and Moller SG. "A Proteomics Approach to Investigate miR-153-3p and miR-205-5p targets in neuroblastoma cells." Plos One. 10 2015: e0143969.

Papaioannou A, Louis M, Dhital B, Ho HP, Chang EJ, Boutis GS. "Quantitative Comparison of Structure and Dynamics of Elastin Following Three Isolation Schemes by 13C Solid State NMR and MALDI Mass Spectrometry." Biochimica et Biophysica Acta. 1854 2015: 391.

Ho H-P, Rathod P, Louis M, Tada CK, Rahaman S, Mark KJ, Leng J, Dana D, Kumar S, Lichterfeld M, Chang EJ. "Studies on Quantitative Phosphopeptide Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry without Label, Chromatography or Calibration Curves." Rapid Communications in Mass Spectrometry. 28 2014: 2681.

Leng J, Ho H-P, Buzon MJ, Pereyra F, Walker BD, Yu XG, Chang EJ, Lichterfeld M. "A Cell-Intrinsic Inhibitor of HIV-1 Reverse Transcription in CD4+ T Cells from Elite Controllers." Cell Host & Microbe. 15 2014: 717.

Basak I, Pal R, Patil KS, Dunne A, Ho H-P, Lee S, Peiris D, Maple-Grodem J, Odell M, Chang EJ, Larsen JP, Moller SG. "Arabidopsis AtPARK13, Which Confers Thermotolerance, Targets Misfolded Proteins." Journal of Biological Chemistry. 289 2014: 14458.

Dana D, De S, Rathod P, Davalos AR, Novoa DA, Paroly S, Torres VM, Afzal N, Lankalapalli RS, Rotenberg SA, Chang EJ, Subramaniam G, Kumar S. "Development of a Highly Potent, Selective and Cell-Active Inhibitor of Cysteine Cathepsin L-A Hybrid Design Approach." Chemical Communications. 50 2014: 10875.

Wilson C, Ramai D, Serjanov D, Lama N, Levinger L, Chang EJ. "Tethered Domains and Flexible Regions in tRNase ZL, the Long Form of tRNaseZ." PLoS ONE. 8 2013: e66942.

Dana D, Davalos AR, De S, Rathod P, Gamage RK, Huestis J, Afzal N, Zavlanov Y, Paroly SS, Rotenberg SA, Subramaniam G, Mark KJ, Chang EJ, Kumar S. "Development of Cell-Active Non-Peptidyl Inhibitors of Cysteine Cathepsins." Bioorganic & Medicinal Chemistry. 21 2013: 2975.

Dana D, Das TK, Kumar I, Daalos AR, Mark KJ, Ramai D, Chang EJ, Talele TT, Kumar S. "Design, Synthesis and Evaluation of 2-(arylsulfonyl)oxiranes as Cell-permeable Covalent Inhibitors of Protein Tyrosine Phosphatases." Chemical Biology and Drug Design. 80 2012: 489.

Westblade LF, Minakhin L, Tackett AJ, Kuznedelov K, Vvedenskaya I, Chang EJ, Wang Q, Mooney RA, Landick R, Chait BT, Severinov K, Darst SA. "Rapid Isolation and Identification of T4 Bacteriophage-Dependent Modifications of Escherichia coli RNA Polymerase using Immunoisolation and MALDI-MS." Journal of Proteome Research. 7 2008: 1244.

Chang EJ, Begum, R Chait BT, Gaasterland T. "Predictive Computational Modeling of Cyclin-Dependent Kinase Substrates." PLoS ONE. 2 2007: e656.

Chang EJ, Archambault V, McLachlin DT, Krutchinsky AN, Chait BT. "Analysis of Protein Phosphorylation by Hypothesis-Driven Multiple-Stage Mass Spectrometry." Analytical Chemistry. 76 2004: 4472.

Archambault V, Chang EJ, Drapkin BJ, Cross FR, Chait BT, Rout MP. "Targeted Proteomic Study of the Cyclin-Cdk Module." Molecular Cell. 14 2004: 699.

Hofmann RM, Cotton GJ, Chang EJ, Vidal E, Veach D, Bornmann W, Muir TW. "Fluorescent Monitoring of Kinase Activity in Real Time: Development of a Robust Fluorescence-Based Assay for Abl Tyrosine Kinase Activity." Bioorganic and Medicinal Chemistry Letters. 11 2001: 3091.

Ha S, Chang E, Lo M, Men H, Park P, Ge M, Walker S. "The Kinetic Characterization of Escherichia coli MurG Using Synthetic Substrate Analogues." Journal of the American Chemical Society. 121 1999: 8415.

Ko L, Ko EC, Nacharaju P, Liu W, Chang E, Kenessey A, Yen SC. "An Immunochemical Study on Tau Glycation in Paired Helical Filaments." Brain Research. 830 1999: 301.

Other

Professional Honors, Prizes, Fellowships

Dean's Award for Undergraduate Research Engagement: 2014.

Provost's Award for Undergraduate Research Engagement: 2013.

Sigma Alpha Pi Excellence in Teaching Award: 2008.

Burroughs-Wellcome Interfaces in Science Award: 1999-2003.

American Institute of Chemists Award: 1999.

Grants-In-Aid

Professional Staff Congress-CUNY, The Role of Protein Phosphorylation in the Proliferation and Pathogenesis of Zika. 7/2017 - 8/2018: $6,000.

Professional Staff Congress-CUNY, A Global Approach to the Discovery of HIV Protein Phosphorylation by Host Cell Kinases. 7/2015 - 6/2016: $6,000.

Professional Staff Congress-CUNY, How is HIV Reverse Transcripatse Regulated by Cyclin Dependent Kianse Phosphorylation?. 7/2014- 6/2015: $3,500.

Professional Staff Congress-CUNY, Regulation of HIV Reverse Transcriptase by Cyclin Dependent Kinase Phosphorylation. 7/2013 - 12/2014: $6,000.

Professional Staff Congress-CUNY Round 42, Cyclin Specificity Using a Novel Mass Spectrometric Quantitative Mulitisite Phosphorylation Assay. 7/2011 - 12/2012: $6,000.

ARRA Supplement to NIH MBRS SCORE Grant, Using isotope labeling/mass spectrometry to probe Cdk1 multisite phosphorylation dynamics. 10/2010 - 6/2012: $160,000.

Collaborative Incentive Research Grant-CUNY, Round 16, Proteomic Analysis of Nup211p. 9/2010 - 8/2012: $60,000.

Luitpold Pharmaceuticals, Pharmaceutical Compound Analysis. 1/2010- 12/2012: $750.

Research Corporation Cottrell College Science Award, Investigating the gas phase chemistry of phosphorylated peptide ions using mass spectrometry. 2/2009 - 1/2011: $45,000.

National Institutes of Health-MBRS/SCORE, Using isotope labeling/mass spectrometry to probe Cdk1 multisite phosphorylation dynamics. 1/2009 - 1/2012: $460,000.

York College Auxiliary Enterprises, Developing Career Paths in Natural Science. 8/2007 - 12/2007: $700.

Professional Staff Congress-CUNY, Round 39, Understanding mass spectrometric phosphopeptide behavior for bioanalytical methods development. 7/2008 - 6/2009: $5,000.

Professional Staff Congress-CUNY, Round 38, Improving the computational prediction of cyclin-dependent kinase phosphorylation substrates. 7/2007 - 6/2008: $5,500.

Research Equipment Grant-CUNY, Probing the dynamics of binding and catalysis in dihydropteridine reductase using a mT-jump stopped-flow device. 3/2007: $40,000.

Graduate Research Technology Initiative-CUNY, Quantitative studies on protein interactions and phosphorylation using isoelectronic focusing and. 9/2006 - 8/2007: $120,000.

Offices Held In Professional Societies

Session organizer, Biological Spectroscopy, 44th Annual Middle Atlantic Regonal Meeting of the American Chemical Society: 5/2015.

Board of Directors, Analytical Topical Group, New York Section of the American Chemical Society: 8/2008 - present.

Session co-organizer, Biological Spectroscopy, 40th Annual Middle Atlantic Regional Meeting of the American Chemical Society, Bayside, NY: 5/2008.