Office Location: AC-3F01G
|Thursday||11:00 AM - 1:00 PM|
|Wednesday||12:00 PM - 1:00 PM|
|PhD||The Rockefeller University||Biochemistry||2006|
Research in the lab of Emmanuel Chang centers around biological applications of mass spectrometry. Our major interest is in analysis and methods development for protein phosphorylation and other post-translational modifications. Applications include phosphorylation in cell cycle; ADP-ribosyltaion; mass spectrometry-based biosensors, and kinase inhibitors and enzyme kinetics. We also collaborate with other CUNY and external scientists who would like to harness the power of mass spectrometry to study there own interesting biological systems.We supplement our experimental work with computational methods including bioinformatic protein sequence analysis and molecular modeling.
Dr. Chang's teaching interests include analytical chemistry, biochemistry, writing and data presentation, literature analysis, and innovations in introductory chemistry.
Areas of Expertise
Chemical and Biochemical
Articles in Field Of Expertise
Papaioannou A, Louis M, Dhital B, Ho HP, Chang EJ, Boutis GS. "Quantitative Comparison of Structure and Dynamics of Elastin Following Three Isolation Schemes by 13C Solid State NMR and MALDI Mass Spectrometry." Biochimica et Biophysica Acta. 1854 2015: 391.
Ho H-P, Rathod P, Louis M, Tada CK, Rahaman S, Mark KJ, Leng J, Dana D, Kumar S, Lichterfeld M, Chang EJ. "Studies on Quantitative Phosphopeptide Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry without Label, Chromatography or Calibration Curves." Rapid Communications in Mass Spectrometry. 28 2014: 2681.
Leng J, Ho H-P, Buzon MJ, Pereyra F, Walker BD, Yu XG, Chang EJ, Lichterfeld M. "A Cell-Intrinsic Inhibitor of HIV-1 Reverse Transcription in CD4+ T Cells from Elite Controllers." Cell Host & Microbe. 15 2014: 717.
Basak I, Pal R, Patil KS, Dunne A, Ho H-P, Lee S, Peiris D, Maple-Grodem J, Odell M, Chang EJ, Larsen JP, Moller SG. "Arabidopsis AtPARK13, Which Confers Thermotolerance, Targets Misfolded Proteins." Journal of Biological Chemistry. 289 2014: 14458.
Dana D, De S, Rathod P, Davalos AR, Novoa DA, Paroly S, Torres VM, Afzal N, Lankalapalli RS, Rotenberg SA, Chang EJ, Subramaniam G, Kumar S. "Development of a Highly Potent, Selective and Cell-Active Inhibitor of Cysteine Cathepsin L-A Hybrid Design Approach." Chemical Communications. 50 2014: 10875.
Wilson C, Ramai D, Serjanov D, Lama N, Levinger L, Chang EJ. "Tethered Domains and Flexible Regions in tRNase ZL, the Long Form of tRNaseZ." PLoS ONE. 8 2013: e66942.
Dana D, Davalos AR, De S, Rathod P, Gamage RK, Huestis J, Afzal N, Zavlanov Y, Paroly SS, Rotenberg SA, Subramaniam G, Mark KJ, Chang EJ, Kumar S. "Development of Cell-Active Non-Peptidyl Inhibitors of Cysteine Cathepsins." Bioorganic & Medicinal Chemistry. 21 2013: 2975.
Dana D, Das TK, Kumar I, Daalos AR, Mark KJ, Ramai D, Chang EJ, Talele TT, Kumar S. "Design, Synthesis and Evaluation of 2-(arylsulfonyl)oxiranes as Cell-permeable Covalent Inhibitors of Protein Tyrosine Phosphatases." Chemical Biology and Drug Design. 80 2012: 489.
Westblade LF, Minakhin L, Tackett AJ, Kuznedelov K, Vvedenskaya I, Chang EJ, Wang Q, Mooney RA, Landick R, Chait BT, Severinov K, Darst SA. "Rapid Isolation and Identification of T4 Bacteriophage-Dependent Modifications of Escherichia coli RNA Polymerase using Immunoisolation and MALDI-MS." Journal of Proteome Research. 7 2008: 1244.
Chang EJ, Begum, R Chait BT, Gaasterland T. "Predictive Computational Modeling of Cyclin-Dependent Kinase Substrates." PLoS ONE. 2 2007: e656.
Chang EJ, Archambault V, McLachlin DT, Krutchinsky AN, Chait BT. "Analysis of Protein Phosphorylation by Hypothesis-Driven Multiple-Stage Mass Spectrometry." Analytical Chemistry. 76 2004: 4472.
Archambault V, Chang EJ, Drapkin BJ, Cross FR, Chait BT, Rout MP. "Targeted Proteomic Study of the Cyclin-Cdk Module." Molecular Cell. 14 2004: 699.
Hofmann RM, Cotton GJ, Chang EJ, Vidal E, Veach D, Bornmann W, Muir TW. "Fluorescent Monitoring of Kinase Activity in Real Time: Development of a Robust Fluorescence-Based Assay for Abl Tyrosine Kinase Activity." Bioorganic and Medicinal Chemistry Letters. 11 2001: 3091.
Ha S, Chang E, Lo M, Men H, Park P, Ge M, Walker S. "The Kinetic Characterization of Escherichia coli MurG Using Synthetic Substrate Analogues." Journal of the American Chemical Society. 121 1999: 8415.
Ko L, Ko EC, Nacharaju P, Liu W, Chang E, Kenessey A, Yen SC. "An Immunochemical Study on Tau Glycation in Paired Helical Filaments." Brain Research. 830 1999: 301.
Presented Papers, Lectures, and Exhibitions and Performances
" Haggagi D, Foster C, Chang E “Molecular Modeling of the Phosphopeptide Ion Fragmentation of Polypeptides in the Gas Phase” Poster. Urban University Conference, Brookhaven, NY " May 2010: .
" Rahaman S, Han K, Marecheau D, Mark KJ, Chang EJ. “Development of a Quantitative in Vitro Cyclin Kinase Assay System and Investigation of Multi-Site Phosphorylation Kinetics” " May 2010: Poster. 58th American Chemical Society Undergraduate Research Symposium, Garden City, NY.
" Lama N, Wilson C, Levinger L, Chang E. “Biophysical Analysis of the Flexible Arm of tRNAseZ” " May 2010: Poster. 58th American Chemical Society Undergraduate Research Symposium, Garden City, NY.
" “A proteomic study of the cyclin-dependent kinase protein network.” " April 2010: Biology Department Seminar, Brooklyn College, Brooklyn, NY.
" Allen-Michaud T, Chang C-H; Profit A, Chang EJ. “A Systematic Study into Gas Phase Phosphopeptide Chemistry” " June 2009: Poster. 57th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA..
" “Identification and Quantification of Protein Phosphorylation Using Hypothesis-Driven Multi-Stage MALDI Mass Spectrometry”. " April 2009: American Chemical Society Analytical Chemistry Focus Group, New York, NY.
" “Move that mass! Mass Spectrometric Analysis of Protein Post-Translational Modifications” " March 2009: Natural Science Seminar Series, York College, Jamaica, NY.
" Chang EJ. "A highly-sensitive, low-cost method for the mass spectrometric analysis of protein phosphorylation." " May 2008: Oral Platform Presentation. 40th annual Middle Atlantic Regional Meeting of the American Chemical Society. Bayside, NY.
" Allen-Michaud, T, Chang EJ. "Studies into the gas phase chemistry of phosphorylated peptide ions using mass spectrometry". " May 2008: Poster. 40th annual Middle Atlantic Regional Meeting of the American Chemical Society. Bayside, NY.
" Cabanas R, Chang EJ. "Substrate specificity in the cyclin-dependent kinase system." " May 2008: Urban University Conference Series 2008. New York, NY.
" Faustin N, Nelson K, Chang EJ. „ Tandem Mass Spectrometric Analysis of Desmosine & Isodesmosine, the Elastin Cross-linking Amino Acids." " May 2008: Urban University Conference Series 2008. New York, NY.
" Allen-Michaud, T, Chang EJ. “Studies into the gas phase chemistry of phosphorylated peptide ions using mass spectrometry.” " May 2008: Urban University Conference Series 2008. New York, NY.
" "Proteomic dissection of the cyclin-dependent kinase protein network." " November 2007: Chemistry Seminar Series. Queens College/CUNY. Flushing, NY, November 2007.
" Bonaparte D, Chang E. "Mass Spectrometric Analysis of ADP-Ribosylation" " October 2007: Poster. Horizons IV Student Research Conference. La Cruces, NM, October 2007.
Professional Honors, Prizes, Fellowships
Sigma Alpha Pi Excellence in Teaching Award: 2008.
Burroughs-Wellcome Interfaces in Science Award: 1999-2003.
American Institute of Chemists Award: 1999.
Professional Staff Congress-CUNY Round 42, Cyclin Specificity Using a Novel Mass Spectrometric Quantitative Mulitisite Phosphorylation Assay. 7/2011 - 12/2012: $6,000.
ARRA Supplement to NIH MBRS SCORE Grant, Using isotope labeling/mass spectrometry to probe Cdk1 multisite phosphorylation dynamics. 10/2010 - 6/2012: $160,000.
Collaborative Incentive Research Grant-CUNY, Round 16, Proteomic Analysis of Nup211p. 9/2010 - 8/2012: $60,000.
Luitpold Pharmaceuticals, Pharmaceutical Compound Analysis. 1/2010- 12/2012: $750.
Research Corporation Cottrell College Science Award, Investigating the gas phase chemistry of phosphorylated peptide ions using mass spectrometry. 2/2009 - 1/2011: $45,000.
National Institutes of Health-MBRS/SCORE, Using isotope labeling/mass spectrometry to probe Cdk1 multisite phosphorylation dynamics. 1/2009 - 1/2012: $460,000.
York College Auxiliary Enterprises, Developing Career Paths in Natural Science. 8/2007 - 12/2007: $700.
Professional Staff Congress-CUNY, Round 39, Understanding mass spectrometric phosphopeptide behavior for bioanalytical methods development. 7/2008 - 6/2009: $5,000.
Professional Staff Congress-CUNY, Round 38, Improving the computational prediction of cyclin-dependent kinase phosphorylation substrates. 7/2007 - 6/2008: $5,500.
Research Equipment Grant-CUNY, Probing the dynamics of binding and catalysis in dihydropteridine reductase using a mT-jump stopped-flow device. 3/2007: $40,000.
Graduate Research Technology Initiative-CUNY, Quantitative studies on protein interactions and phosphorylation using isoelectronic focusing and. 9/2006 - 8/2007: $120,000.
Offices Held In Professional Societies
Board of Directors, Analytical Topical Group, New York Section of the American Chemical Society: 8/2008 - present.
Session co-coordinator, Biological Spectroscopy, 40th Annual Middle Atlantic Regional Meeting of the American Chemical Society, Bayside, NY: 5/2008.