Office Location: AC-3F01B
|PhD||SUNY Stony Brook||Chemistry||1997|
|BS||Lehman College of CUNY||Chemistry|
Currently there are two main areas of investigation being pursued in my laboratory.
1) Amyloid Formation and Protein Aggregation. The abnormal formation of protein aggregates, or amyloid deposits, is the hallmark of Alzheimer’s disease as well as Type 2 diabetes. My laboratory is investigating the molecular interactions that occur between key proteins that contribute to the formation of amyloid in these diseases. Through a more detailed understanding of how these proteins self-assembly to form aggregates, we hope to design and develop inhibitors which may serve as a template for potential therapeutic agents.
2) Protein Kinase Inhibitors. We are developing compounds that inhibit the activity of key enzymes (kinases) which can cause tissues to grow out of control and develop into tumors. To do this we are synthesizing molecules that exploit the unique molecular recognition motifs found in these enzymes to more effectively deliver inhibitory species to the active site.
Areas of Expertise
- Protein aggregation and amyloid formation
Design and development of kinase inhibitors and multivalent ligands
Peptide and peptoid synthesis
Articles in Field Of Expertise
Profit, A. A. and Desamero, R. Z. B.. "Development of Peptide-Based Inhibitors of Amylin Aggregation Employing Aromatic and Electrostatic Repulsion." Rational Drug Design: Methods and Protocols. In Press 2/2/2018: .
Lagarias, P., Elkhou, Y., Vedad, J., Profit, A., Kellici, T. F., Kolocouris, A. Desamero, R., and Mavromoustakos, T.. "Molecular dynamics simulations on the bioactive molecule hIAPP22-29 (NFGAILSS) and rational drug design." Rational Drug Design: Methods and Protocols. In Press 2/2/2018: .
Profit, A. A., Vedad, J. and Desamero, R. Z. B.. "Peptide Conjugates of Benzene Carboxylic Acids as Agonists and Antagonists of Amylin Aggregation." Bioconjugate Chem.. 28 2017: 666-677.
Vedad, J., Domaradzki, M., Mojica, E.-R., Chang, E. J., Profit, A. A. and Desamero,R. Z. B.. "Conformational Differentiation of α-Cyanohydroxycinnamic Acid Isomers: A Raman Spectroscopic Study." Journal of Raman Spectrosopy. 48 2017: 1282-1288.
Wang, Y., Dehigaspitiya, D. C., Levine, P. M., Profit, A. A., Haugbro, M., Imberg-Kazdan, K., Logan, S. K.. "Multivalent Peptoid Conjugates Which Overcome Enzalutamide Resistance in Prostate Cancer Cells." Cancer Res. 76 2016: 5124-5132.
Profit, A. A., Vedad, J. Saleh, M., and Desamero, R. Z. B.. "Aromaticity and Amyloid Formation: Effect of pi-Electron Distribution and Aryl Substituent Geometry on the Self-Assembly of Peptides Derived from hIAPP22-29." Arch Biochem Biophys. 567 2015: 46-58.
Profit, A.A., Felsen, V., Chinwong, J., E.-R., Mojica and Desamero, R. Z. B.. "Evidence of pi-Stacking Interactions in the Self-Assembly of hIAPP22-29." PROTEINS: Structure, Function and Bioinformatics. 81 2013: 690-703.
Desamero, R. Z. B., Kang, J., Dol, C., Chingwong, J.,Walters, K., Sivarajah, T. and Profit A. A. "Spectroscopic Characterization of the SH2- and Active Site-Directed Peptide Sequences of a Bivalent Src Kinase Inhibitor." Applied Spectroscopy. 63 2009: 767-774.
Profit, A. A., Lee, T. R., Niu, J. and Lawrence, D. S. "Molecular Rulers: An Assessment of Distance and Spatial Relationships of Src Tyrosine Kinase SH2 and Active Site Regions." Journal of Biological Chemistry. 276 2001: 9446-9451.
Profit, A. A., Lee, T. R. and Lawrence, D. S. "Bivalent Inhibitors of Protein Tyrosine Kinases." Journal of the American Chemical Society. 121 1999: 280-283.
Sims, B., Nahnke-Zizelman, D. K., Profit, A. A., Prestwich, G. D., Sabina, R. L. and Theibert, A. B. "Regulation of AMP Deaminase by Phosphoinositides." Journal of Biological Chemistry. 274 1999: 25701-25707.
Blader, I. J., Cope, M. J., Jackson, T. R., Profit, A. A., Greenwood, A. F., Drubin, D. G., Prestwich, G. D. and Theibert, A. B.. "GCS1, an Arf GAP in Saccharomyces Cerevisiae is Required for Normal Actin Cytoskeletal Organization In Vivo and Stimulated Actin Polymerization In Vitro." Molecular Biology of the Cell. 10 1999: 581-596.
Profit, A. A., Chen, J., Gu, Q. M., Chaudhary, A., Prasad, K., Lafer, E. M. and Prestwich, G. D. "Probing the Phosphoinositide Binding Site of the Clathrin Assembly Protein AP-2 with Photoaffinity Labels." Archives of Biochemistry and Biophysics. 357 1998: 85-94.
Chaudhary, A., Gu, Q.-M., Thum, O., Profit, A., Qing, Y., Jeyakumars, L., Fleischer, S. and Prestwich, G. D. "Specific Interaction of Golgi Coatomer COP with Phosphatidylinositol (3,4,5)-Trisphosphate." Journal of Biological Chemistry. 273 1998: 8344-8350.
Tall, E., Dormán, G., Garcia, P., Runnels, L., Shah, S., Chen, J., Profit, A. A., Gu, Q.-M., Chaudhary, A., Prestwich, G. D. and Rebecchi, M. J. "Phosphoinositide Binding Specificity among Phospholipase C Isozymes as Determined by Photo-Cross-Linking to Novel Substrate and Product Analogs." Biochemistry. 36 1997: 7239-7248.
Mehotra, B., Elliot, J. T., Chen, J., Olszewski, J. D., Profit, A. A., Chaudhary, A., Fukuda, M., Mikoshiba, K. and Prestwich, G. D. "Selective Photoaffinity Labeling of Inositol Polyphosphate Binding C2B Domains of Synaptotagmins." Journal of Biological Chemistry. 272 1997: 4237-4244.
Chen, J., Profit, A. A., and Prestwich, G. D. "Synthesis of Photoactivatable 1,2-O-Diacyl-sn-glycerol Derivatives of 1-L-Phosphatidyl-D-myo-inositol 4,5-Bisphosphate (PtdInsP2) and 3,4,5-Trisphosphate (PtdInsP3)." Journal of Organic Chemistry. 61 1996: 6305-6312.
Hammonds-Odie, L. P., Jackson, T. R., Profit, A. A., Blader, I. J.,Turck, C.,Prestwich, G. D., and Theibert, A. B. "Identification and Cloning of Centaurin-: a Novel Phosphatidylinositol (3,4,5)-Trisphosphate Binding Protein from Rat Brain." Journal of Biological Chemistry. 271 1996: 18859-18868.
Osapay, G., Profit, A., Taylor, J. W. "Synthesis of Tyrocidine A: Use of Oxime Resin for Peptide Chain Assembly and Cyclization." Tetrahedron Lett. 31 1990: 6121-6124.
Richards, L., Tai, C., Ytuarte, L., Knights, M., Richards, J. L., Profit, A., Mirsky, S., McNamee, E. "A Soluble 5-Phenyltetrazolate-bridged Rhodium (III) Oligomer." Inorganica Chimica Acta. 156 1989: 33-35.
Refereed Proceedings in Field Of Expertise
Osapay, G., Gulyas, J., Profit, A., Gulyas, E. S., Taylor, J. W. "Multicyclic Peptides Synthesized Using Oxime Resin: Helix Stabilizing Effects of Lactam Bridges." Peptides: Chemistry and Biology. Proceedings of the12th American Peptide Symposium. 1992: pp 239-240.
Presented Papers, Lectures, and Exhibitions and Performances
" Development of Peptoid-Based PROTACs Targeting the Androgen Receptor " 3/22/17: NYU Biomedical Chemistry Institute (Kirshenbaum group).
Professional Honors, Prizes, Fellowships
UNCF•Merck Postdoctoral Fellow: 1998.
National Science Foundation Predoctoral Assistantship: 1991.
Exxon Predoctoral Fellowship: 1991.
National Institute of Health, Probing the role of helix-1 in amyloid formation by serum amyloid A. 2016-19: $493,205.
National Institute of Health (NIH), Exploration of Bivalent Inhibitors of the Akt Kinase. 2006-09: $346,852.
Research Corporation Cottrell College Science Award, Peptide Conjugate Inhibitors of Protein Kinase B. 2006-09: $35,684.
PSC-CUNY 39, Benzene 1,2,3,4-tetrakisphosphate Conjugates as Inhibitors of Protein Kinase B. 2008-09: $4,500.
PSC-CUNY 37, Structural Optimization of Bivalent Src Kinase Inhibitors. 2005-07: $4,000.
PSC-CUNY 36, Structural Optimization of Bivalent Src Kinase Inhibitors. 2005-06: $4,959.
Other Professional Activities and Public Service
Manuscript review for the American Chemical Society: 2017.
Manuscript review for the Royal Society of Chemistry: 2016.