Chang, Emmanuel
- Overview
Assistant Professor
Chemistry
Phone: (718) 262-3778
Office Location: AC-3F01
echang@york.cuny.edu
| Office Hours | |
|---|---|
| Monday, Wednesday | 10:30-11:50 AM |
| or by appointment |
- Education:
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Degree Institution Field Dates PhD The Rockefeller University Biochemistry 2006 BA Princeton University Chemistry 1999
Research in the lab of Emmanuel Chang centers around biological applications of mass spectrometry. Our major interest is in analysis and methods development for protein phosphorylation and other post-translational modifications. Applications include phosphorylation in cell cycle; ADP-ribosyltaion; mass spectrometry-based biosensors, and kinase inhibitors and enzyme kinetics. We also collaborate with other CUNY and external scientists who would like to harness the power of mass spectrometry to study there own interesting biological systems.
The lab is equipped with MALDI-TOF and electrospray qTOF mass spectrometers from Waters Corporation, and a vMALDI LTQ linear ion from Thermo. Ancillary equipement includes a Waters nanoAcquity UPLC, and molecular biology, microbial cell culture and protein purification apparatuses. We supplement our experimental work with computational methods including bioinformatic protein sequence analysis and molecular modeling.
Dr. Chang's teaching interests include analytical chemistry, biochemistry, writing and data presentation, literature analysis, and innovations in introductory chemistry.
- Areas of Expertise:
- Biochemistry
Analytical Chemistry
Mass Spectrometry
Proteomics
Bioinformatics
Phosphorylation
Cell Cycle
Chemical and Biochemical Education
- Articles in Field Of Expertise:
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Westblade LF, Minakhin L, Tackett AJ, Kuznedelov K, Vvedenskaya I, Chang EJ, Wang Q, Mooney RA, Landick R, Chait BT, Severinov K, Darst SA. "Rapid isolation and identification of T4 bacteriophage-dependent modifications of Escherichia coli RNA polymerase using immunoisolation and MALDI-MS." Journal of Proteome Research. 7 2008: 1244.
Chang EJ, Begum, R Chait BT, Gaasterland T. "Predictive computational modeling of cyclin-dependent kinase substrates." PLoS ONE. 2 2007: e656.
Chang EJ, Archambault V, McLachlin DT, Krutchinsky AN, Chait BT. "Analysis of Protein Phosphorylation by Hypothesis-Driven Multiple-Stage Mass Spectrometry." Analytical Chemistry. 76 2004: 4472.
Archambault V, Chang EJ, Drapkin BJ, Cross FR, Chait BT, Rout MP. "Targeted Proteomic Study of the Cyclin-Cdk Module." Molecular Cell. 14 2004: 699.
Hofmann RM, Cotton GJ, Chang EJ, Vidal E, Veach D, Bornmann W, Muir TW. "Fluorescent monitoring of kinase activity in real time: development of a robust fluorescence-based assay for Abl tyrosine kinase activity." Bioorganic and Medicinal Chemistry Letters. 11 2001: 3091.
Ha S, Chang E, Lo M, Men H, Park P, Ge M, Walker S. "The Kinetic Characterization of Escherichia coli MurG Using Synthetic Substrate Analogues." Journal of the American Chemical Society. 121 1999: 8415.
Ko L, Ko EC, Nacharaju P, Liu W, Chang E, Kenessey A, Yen SC. "An Immunochemical Study on Tau Glycation in Paired Helical Filaments." Brain Research. 830 1999: 301.
- Professional Honors, Prizes, Fellowships:
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Sigma Alpha Pi Excellence in Teaching Award: 2008.
Burroughs-Wellcome Interfaces in Science Award: 1999-2003.
American Institute of Chemists Award: 1999.
- Grants-In-Aid:
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Collaborative Incentive Research Grant-CUNY, Quantitative studies on protein interactions and phosphorylation using isoelectronic focusing and. : $60,000.
Research Corporation Cottrell College Science Award, Investigating the gas phase chemistry of phosphorylated peptide ions using mass spectrometry. 2/2009-1/2011: $45,000.
National Institutes of Health-MBRS/SCORE, Using isotope labeling/mass spectrometry to probe Cdk1 multisite phosphorylation dynamics. 1/2009-1/2012: $460,000.
Professional Staff Congress-CUNY, Understanding mass spectrometric phosphopeptide behavior for bioanalytical methods development. 7/2008-6/2009: $5,000.
Professional Staff Congress-CUNY, Improving the computational prediction of cyclin-dependent kinase phosphorylation substrates. 7/2007-6/2008: $5,000.
Research Equipment Grant-CUNY, Probing the dynamics of binding and catalysis in dihydropteridine reductase using a mT-jump stopped-flow device. 3/2007: $40,000.
Graduate Research Technology Initiative-CUNY, Quantitative studies on protein interactions and phosphorylation using isoelectronic focusing and. 9/2006-8/2007: $120,000.
