NSSS: Structure of histone mRNA stem-loop by Dazhi Tan
Nov 21, 2013
from 12:00 PM to 01:00 PM
|Contact Name||Yolanda Small|
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Dazhi Tan Graduate Student in the Laboratory of Liang Tong Department of Biology, Columbia University, New York, NY, 10027
Molecular basis for the recognition of stem-loops in eukaryotic mRNAs
Stem-loops are the most basic building blocks of more complicated RNA tertiary structures. They exist ubiquitously in the untranslated regions of eukaryotic mRNAs, and are crucial for the post-transcriptional regulation of gene expression. The elucidation of the molecular basis for the recognition of such stemloop structures is thus of great significance for understanding how the stem-loops are capable of controlling transcript stability, alternative splicing, RNA localization, etc. With the powerful tool of X-ray crystallography, we have obtained the high-resolution structures of two protein-RNA complexes containing mRNA stem-loops.
One is the histone mRNA stem-loop, stem-loop binding protein (SLBP) and 3'hExo ternary complex that is the central regulator of histone mRNA metabolism. The other includes the constitutive decay element (CDE) of TNFa and Roquin, and is critical for containing autoimmunity and inflammation. This talk will focus on the two distinct stem-loop recognition modes we observed in these two complexes, as well as how they reflect the functions of these complexes.