Gene Silencing by Human tRNase ZL

Minority Access to Research Careers (MARC, a NIH-funded Training Program) and The Science Departments Present a Research Seminar by Masayuki Nashimoto NUPALS, Japan
When Feb 01, 2008
from 02:00 PM to 03:15 PM
Where Chemistry Conference Room 3F01g
Contact Name
Contact Phone 718-262-2704
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Gene Silencing by Human tRNase ZL

Under the Direction of Small Guide RNA

The long form of human tRNA 3′processing endoribonuclease (tRNase ZL) can cleave any target RNA at any desired site by recognizing a pre-tRNA-like or micro-pre-tRNA-like complex formed between the target RNA and artificial small guide RNA (sgRNA). Using this property, we can downregulate gene expression in the cells by introducing sgRNAs either as their expression plasmids or as 2′-O-methyl RNAs.

This technology is called TRUE gene silencing after tRNase ZL-utilizing efficacious gene silencing. Recently, we found that human cytosolic tRNase ZL modulates gene expression through various types of cellular small noncoding RNA (ncRNA). It is now clear that the reason why TRUE gene silencing works well in the cellsis because it utilizes artificial sgRNAs like naturally occurring ncRNAs and mimics the intrinsic cellular gene regulatory system.